|
KMID : 0914820030030040206
|
|
Journal of the Korean Gastric Cancer Association
2003 Volume.3 No. 4 p.206 ~ p.213
|
|
|
p53 Gene Mutation, Tumor p53 Protein Over expression, and Serum Anti-p53 Antibody in Patients with Gastric Cancer
|
|
Bong Jin-Gu
Lee Myung-Hoon
Song Kyung-Eun
Kim Tae-Bong
Yu Wan-Sik
|
|
|
Abstract
|
|
|
Purpose: The clinical implication of p53 mutation in gastric cancer is still unclear, as shown by the discordant results that continue to be reported in the literature.
Materials and Methods: To assess p53 gene mutation, tumor p53 overexpression, and serum anti-p53 antibody, we employed a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis, an immunohistochemistry using monoclonal antibody DO-7, and an enzyme- linked immunosorbent assay (ELISA), respectively.
Results: Of 169 surgical specimens of gastric cancer, mutation at exon 5∼8 of the p53 was identified in 33 (19.5%) and was significantly correlated with lymph node metastasis. Overexpression of p53 was found in 62 specimens (36.7%) and had a significant correlation with tumor differentiation. Serum anti-p53 antibody was positive in 18 patients (10.7%). Twenty-three of the mutated tumors (69.7%) and 39 of the non-mutated tumors (28.7%) displayed immunoreactivity. Twelve of the immunopositive tumors (19.4%) and 6 of the immunonegative tumors produced anti-p53 antibody. These differences were statistically significant (P＜0.001 and P=0.005, respectively). There was no significant difference in survival according to the mutation of p53.
Conclusion: Mutation and overexpression of p53 can be easily detected by immunohistochemistry. However, standardi- zation of the immunohistochemical staining method, as well as guidelines for interpreting the stained result, will produce concordant results and thereby improve clinical application.
|
|
|
KEYWORD
|
|
|
Gastric cancer, p53 mutation, p53 overexpression, anti-p53 antibody
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|